Qualifications & Memberships
Research in our lab focuses on the structure, function and inhibition of key enzymes involved in infection and disease. We are also interested in the evolution of enzyme function, and conversely its design and manipulation.
We study a variety of enzymes: from those responsible for the biosynthesis of amino acids, in particular lysine, since these are validated drug targets; to glycolytic enzymes, such as pyruvate kinase, since these are tractable model systems to understand enzyme allostery. The list is ever increasing.
A common theme in the lab is the role protein-protein interactions in a variety of biological systems, mostly derived from bacteria, but more recently including eukaryotic proteins involved in cancer, where we hope to address the twin problems of how and why proteins form complexes and the functional consequences of these associations.
- Horne CR., Venugopal H., Panjikar S., Wood DM., Henrickson A., Brookes E., North RA., Murphy JM., Friemann R. and Griffin MDW. (2021) Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism.. Nat Commun 12(1): 1988. http://dx.doi.org/10.1038/s41467-021-22253-6.
- Klionsky DJ., Abdel-Aziz AK., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard MH., Abudu YP. and Acevedo-Arozena A. (2021) Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy http://dx.doi.org/10.1080/15548627.2020.1797280.
- Parthasarathy A., Borrego EJ., Savka MA., Dobson RCJ. and Hudson AO. (2021) Amino acid-derived defense metabolites from plants: A potential source to facilitate novel antimicrobial development.. J Biol Chem : 100438. http://dx.doi.org/10.1016/j.jbc.2021.100438.
- Adams LE., Rynkiewicz P., Babbitt GA., Mortensen JS., North RA., Dobson RCJ. and Hudson AO. (2020) Comparative Molecular Dynamics Simulations Provide Insight Into Antibiotic Interactions: A Case Study Using the Enzyme L,L-Diaminopimelate Aminotransferase (DapL). Frontiers in Molecular Biosciences 7 http://dx.doi.org/10.3389/fmolb.2020.00046.
- Coombes D., Davies JS., Newton-Vesty MC., Horne CR., Setty TG., Subramanian R., Moir JWB., Rosmarie Friemann X., Panjikar S. and Griffin MDW. (2020) The basis for non-canonical ROK family function in the N-acetylmannosamine kinase from the pathogen Staphylococcus aureus. Journal of Biological Chemistry 295(10): 3301-3315. http://dx.doi.org/10.1074/jbc.RA119.010526.