Qualifications & Memberships
Research in our lab focuses on the structure, function and inhibition of key enzymes involved in infection and disease. We are also interested in the evolution of enzyme function, and conversely its design and manipulation.
We study a variety of enzymes: from those responsible for the biosynthesis of amino acids, in particular lysine, since these are validated drug targets; to glycolytic enzymes, such as pyruvate kinase, since these are tractable model systems to understand enzyme allostery. The list is ever increasing.
A common theme in the lab is the role protein-protein interactions in a variety of biological systems, mostly derived from bacteria, but more recently including eukaryotic proteins involved in cancer, where we hope to address the twin problems of how and why proteins form complexes and the functional consequences of these associations.
- Arif T., Currie MJ., Dobson RCJ., Newson HL., Poonthiyil V., Fairbanks AJ., North RA. and Rendle PM. (2021) Synthesis of N-acetylmannosamine-6-phosphate derivatives to investigate the mechanism of N-acetylmannosamine-6-phosphate 2-epimerase. Carbohydrate Research 510 http://dx.doi.org/10.1016/j.carres.2021.108445.
- Crowther JM., Gilmour LH., Porebski BT., Heath SG., Pattinson NR., Owen MC., Fredericks R., Buckle AM., Fee CJ. and Göbl C. (2021) Molecular basis of a redox switch: Molecular dynamics simulations and surface plasmon resonance provide insight into reduced and oxidised angiotensinogen. Biochemical Journal 478(17): 3319-3330. http://dx.doi.org/10.1042/BCJ20210476.
- Currie MJ., Manjunath L., Horne CR., Rendle PM., Subramanian R., Friemann R., Fairbanks AJ., Muscroft-Taylor AC., North RA. and Dobson RCJ. (2021) N-acetylmannosamine-6-phosphate 2-epimerase uses a novel substrate-assisted mechanism to catalyze amino sugar epimerization. Journal of Biological Chemistry 297(4) http://dx.doi.org/10.1016/j.jbc.2021.101113.
- Davies JS., Currie MJ., Wright JD., Newton-Vesty MC., North RA., Mace PD., Allison JR. and Dobson RCJ. (2021) Selective Nutrient Transport in Bacteria: Multicomponent Transporter Systems Reign Supreme. Frontiers in Molecular Biosciences 8 http://dx.doi.org/10.3389/fmolb.2021.699222.
- Horne CR., Venugopal H., Panjikar S., Wood DM., Henrickson A., Brookes E., North RA., Murphy JM., Friemann R. and Griffin MDW. (2021) Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism. Nature Communications 12(1) http://dx.doi.org/10.1038/s41467-021-22253-6.