Seminar Series

Keeping Things Intact: Adventures in Mass Spectrometry


Tim Allison


Chemistry Research Laboratory Department of Chemistry, University of Oxford

Time & Place

Fri, 19 Feb 2016 12:00:00 NZDT in Room 531

All are welcome


Specialised developments in mass spectrometry have led to the field of intact (or native) mass spectrometry, which has become increasingly popular for probing protein structure and interactions. This technique uses soft ionisation techniques, and adjustments to instrument pressures and electronics to enable proteins to be measured simultaneous with preserving noncovalent interactions, such as those between subunits, or with small molecules. Here, with emphasis on membrane proteins, I will briefly outline how this is done, what we're currently doing to refine our understanding of what happens to these types of ions in the gas phase, and how application of this technology is emerging as a useful tool in probing the structure and function of this intractable class of protein.