Seminar Series

A Microfluidic Gradient Generator to Simulate the Oxygen Microenvironment in Cancer Cell Culture

Speaker

Louise Orcheston-Findlay, PhD Candidate

Institute

Department of Electrical and Computer Engineering

Time & Place

Fri, 20 Oct 2017 14:00:00 NZDT in E14, Engineering Core block

Abstract

Standard cancer lab models lack many attributes of the in-vivo cancer microenvironment. Oxygen levels for example are not commonly controlled in 2D cell-culture well plate experiments. However, low oxygen (hypoxia) in particular is common in cancerous tissue due to high proliferation rates of cancer cells and inadequate tumour vasculature. Hypoxia is also associated with the development of aggressive phenotypes, cancer recurrence and drug resistance. We report a microfluidic system capable of exposing a 2D cellculture to a dissolved oxygen gradient ranging from hypoxia (<1 mg/L) to hyperoxia (40 mg/L) for over 30 minutes, measurable in real-time using an integrated sensor film. The film incorporates a photostable, noncytotoxic oxygen-sensitive fluorescent dye, which exhibits a linear response and high contrast (I0/I100=12) within the range of interest, was integrated onto glass substrates as a cell culture substrate. To demonstrate the applicability of the platform, Ishikawa human endometrial cancer cells were cultured on the platform and exposed to linear cross-stream oxygen gradients. The platform provides a valuable tool for the culture of cancer cells in an in-vivo like microenvironment and will enable more accurate screening of newly discovered anti-cancer drugs.