Qualifications & Memberships
Research in our lab focuses on the structure, function and inhibition of key enzymes involved in infection and disease. We are also interested in the evolution of enzyme function, and conversely its design and manipulation.
We study a variety of enzymes: from those responsible for the biosynthesis of amino acids, in particular lysine, since these are validated drug targets; to glycolytic enzymes, such as pyruvate kinase, since these are tractable model systems to understand enzyme allostery. The list is ever increasing.
A common theme in the lab is the role protein-protein interactions in a variety of biological systems, mostly derived from bacteria, but more recently including eukaryotic proteins involved in cancer, where we hope to address the twin problems of how and why proteins form complexes and the functional consequences of these associations.
- Coombes D., Davies JS., Newton-Vesty MC., Horne CR., Setty TG., Subramanian R., Moir JWB., Friemann R., Panjikar S. and Griffin MDW. (2020) The basis for non-canonical ROK family function in the N-acetylmannosamine kinase from the pathogen Staphylococcus aureus.. J Biol Chem http://dx.doi.org/10.1074/jbc.RA119.010526.
- Love MJ., Abeysekera GS., Muscroft-Taylor AC., Billington C. and Dobson RCJ. (2020) On the catalytic mechanism of bacteriophage endolysins: Opportunities for engineering. Biochimica et Biophysica Acta - Proteins and Proteomics 1868(1) http://dx.doi.org/10.1016/j.bbapap.2019.140302.
- Nouchikian L., Lento C., Donovan K., Dobson R. and Wilson DJ. (2020) Comparing the Conformational Stability of Pyruvate Kinase in the Gas Phase and in Solution.. J Am Soc Mass Spectrom.
- Coombes D., Moir JWB., Poole AM., Cooper TF. and Dobson RCJ. (2019) The fitness challenge of studying molecular adaptation. Biochemical Society Transactions 47(5): 1533-1542. http://dx.doi.org/10.1042/BST20180626.
- Crowther JM., Cross PJ., Oliver MR., Leeman MM., Bartl AJ., Weatherhead AW., North RA., Donovan KA., Griffin MDW. and Suzuki H. (2019) Structure-function analyses of two plant mesodiaminopimelate decarboxylase isoforms reveal that active-site gating provides stereochemical control. Journal of Biological Chemistry 294(21): 8505-8515. http://dx.doi.org/10.1074/jbc.RA118.006825.