Mitja Remus-Emsermann

LecturerMitja Remus-Emsermann

Microbiology
Internal Phone: 95351
http://www.remus-emsermann-lab.org

Qualifications & Awards

Research Interests

I am a microbiology lecturer/ assistant professor at the School of Biological Sciences and associate investigator at the Biomolecular Interaction Centre (BIC), University of Canterbury, New Zealand, focussing on microbial ecology, specifically on plant-microbe interactions and investigation of bacteria at the single-cell resolution.

In my lab, we are mainly interested how bacterial species grow on plant leaves (the phyllosphere), which growth pattern they exhibit and how they interact with other species that share the leaf with them. To do so, we mostly rely on fluorescence microscopy techniques such as confocal laser scanning microscopy and widefield epifluorescence microscopy. This in combination with bacteria that are either tagged with fluorescent protein genes, stained using fluorescence in situ hybridisation (FISH), or stained using fluorescent live/dead stains that allow to determine if individual bacterial cells are either alive or dead. To analyse our data we employ image cytometry and spatial statistic tools which allow us to assess pair aggregation patterns of leaf colonising bacteria.

Since many leaf colonising bacteria cannot be used with the molecular toolbox available for E. coli and other model bacteria, a lot of research time in my lab is dedicated to adapt existing tools to make environmental bacteria more tractable to genetic manipulation and to effectively "make them shine".

Recent Publications

  • Remus-Emsermann MNP. (2018) Leaf surface microbial ecology at the bacterial individual resolution – understanding bacteria one cell at a time. University Auckland: NZMEC5, 14 Apr 2018.
  • Remus-Emsermann MNP. and Schlechter RO. (2018) Phyllosphere microbiology: at the interface between microbial individuals and the plant host. New Phytologist 218(4): 1327-1333. http://dx.doi.org/10.1111/nph.15054.
  • Remus-Emsermann MNP., Pelludat C., Gisler P. and Drissner D. (2018) Conjugation dynamics of self-transmissible and mobilisable plasmids intoE. coliO157:H7 onArabidopsis thalianarosettes. http://dx.doi.org/10.1101/375402.
  • Schmid M., Frei D., Patrignani A., Schlapbach R., Frey JE., Remus-Emsermann MNP. and Ahrens CH. (2018) Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats.. Nucleic Acids Res http://dx.doi.org/10.1093/nar/gky726.
  • Schmid M., Frei D., Patrignani A., Schlaplach R., Frey JE., Remus-Emsermann MNP. and Ahrens CH. (2018) Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats. http://dx.doi.org/10.1101/300186.