Callaghan Innovation's Protein Science and Engineering Team
The Protein Science and Engineering team, co-located within BIC, is part of the Biotechnologies group of Callaghan Innovation.
They provide biotechnology R&D services to help customers create opportunities through innovation. Their expert team of scientists work closely with clients to design and deliver tailored solutions for biotechnology research. Contact them to discuss the best ways for you to develop your ideas.
● Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Bacillus subtilis), yeast (Pichia pastoris) and mammalian (HEK293) expression
● Regulatory approval and access to fermentation capabilty for expression of proteins and biological materials in all common microbial species up to 1000-L scale
● Suite of expression vectors, chaperones, and strains
● Parallel expression of protein fusions for purification, detection and solubility
● Fusion tag removal with HRV3C, TEV and SUMO proteases
● Antibody engineering for production of recombinant antibodies, nanobodies, light chains, chimeric mouse/human antibodies, Fv and Fab antibody fragments, recombinant immune complexes and polymeric IgGs
● Hybridoma maintenance and cloning
● Antibody purification and isotyping
● Epitope mapping using random epitope and peptide libraries
● Isolation and recombinant expression of antibody genes from hybridomas
● In-house modular DNA assembly platform
● Combinatorial gene assembly from libraries of promoters, terminators, etc.
● Multigene assembly
● Vector design and construction
● Metabolic pathway construction
● Site directed mutagenesis
● PCR and DNA sequencing
● Quantitative PCR
● DNA and RNA extraction
● Fluorometric high sensitivity DNA and RNA quantification
● Agarose gel electrophoresis
● Cell fractionation
● Fast protein liquid chromatography (FLPC) including affinity chromatography, ion exchange chromatography and size exclusion chromatography (SEC)
● Hydrophobic interaction chromatography (HIC)
● Protein refolding
● Desalting
● Freeze drying
● Protein electrophoresis (SDS-PAGE)
● Isoelectric focusing (IEF)
● Western blotting
● Protein concentration measurement by bicinchoninic acid, Lowry, Bradford and UV
● Structural integrity by circular dichroism (CD)
● Thermal stability by differential scanning calorimetry (NanoDSC)
● Biomolecular interactions by isothermal calorimetry (ITC)
● Mass spectroscopy (total mass and peptide mapping)
● Kinetic analysis of biomolecular interactions using surface plasmon resonance and bio-layer interferometry
● X-ray crystallography
● Particle size analysis by light scattering and analytical ultracentrifugation
● Spectroscopy (far UV, near UV, infrared, fluorescence)
● Gas chromatography(GC-FID, GC-MS)
● High performance liquid chromatography (HPLC/UPLC-variable wavelength/CAD)
● Nuclear magnetic resonance (1D and 2D)
● Mass spectroscopy
● Enzyme and coupled enzyme assays (colourimetric, UV, fluorescence, luminescence)
● High performance liquid chromatography
● ELISA (direct, indirect, sandwich, competition & inhibition) for protein quantitation and study of protein-protein interactions
● Zeta potential
● Endotoxin measurement
● Chemical quantitation assays
● High performance liquid chromatography (HPLC/UPLC-variable wavelength/CAD)
● Cytotoxicity
● Anti-inflammatory assays (cytochrome oxidase and lipoxygenase, prostaglandin ELISA suite)
● Confocal Microscopy
● Reporter gene assays
In addition to R&D services, we also offer
● Scientific consultancy
● Literature and patent reviews
● Project management
● Industry placement and technology transfer
● Coordination of access to complementary research services
Find out more about our team and range of expertise, or contact us.