UC Spark

Professor Emily Jane Parker

Chemistry; Biomolecular Interaction Centre

Researcher Summary

Research is in the area of biochemistry, particularly the interactions between biological molecules.

Subject Area: Disciplines

Research Groups

Publications


  • Reichau, S. and Parker, E.J. (2013) Active Site Plasticity of a Critical Enzyme from Mycobacterium tuberculosis. RSC Advances 3: 3209-3212. http://dx.doi.org/10.1039/c3ra00037k. (Journal Article)
  • Huisman, F.M.H., Squire, C.J. and Parker, E.J. (2013) Amino-acid substitutionsat the domain interface affect substrate and allosteric inhibitor binding in alpha-isopropylmalate synthase from Mycobacterium tuberculosis. Biochemical and Biophysical Research Communications dx.doi.org/10.1016/j.bbrc.2013.02.092. (Journal Article)
  • Joseph, D.D. Jiao., W. and Parker E.J. (2013) Arg314 is essential for catalysis by N-acetyl neuraminic acid synthase from Neisseria meningitidis. Biochemistry http://dx.doi.org/10.1021/bi400062. (Journal Article)
  • Cross, P.J., Allison, T.M., Dobson, R.C.J., Jameson, G.B. and Parker, E.J. (2013) Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain. Proceedings of the National Academy of Sciences of the United States of America 110(6): 2111-2116. (Journal Article)
  • Xiuming Xiong, Zhijian Xu., Zhuo Yang, Yingtao Liu., Di Wang, Mei Dong., Emily J. Parker. and Weiliang Zhu. (2013) Key Targets and Relevant Inhibitors for the Drug Discovery of Tuberculosis. Current Drug Targets 14 1389-4501/13. (Journal Article)
  • Castell, A., Short, F. L., Evans, G. L., Cookson, T. V. M., Bulloch, E. M. M., Joseph, D. D. A., Lee, C. E., Parker, E. J., Baker, E. N. and Lott, J. S. (2013) Substrate capture mechanism of Mycobacterium tuberculosis anthranilate phosphoribosyltransferase provides a mode for inhibition. Biochemistry 52: 1776-1787.. http://dx.doi.org/10.1021/bi301387m. (Journal Article)
  • Blackmore, N. J., Reichau, S., Jiao, W., Hutton, R. D., Baker, E. N., Jameson, G. B. and Parker, E. J. (2013) Three sites and you are out: Ternary synergistic allostery controls aromatic amino acid biosynthesis in Mycobacterium tuberculosis. Journal of Molecular Biology http://dx.doi.org/10.1016/j.jmb.2012.12.019.. (Journal Article)
  • Jiao, W., Hutton, R.D., Cross, P.J., Jameson, G.B. and Parker, E.J. (2012) Dynamic cross-talk among remote binding sites: The molecular basis for unusual synergistic allostery. Journal of Molecular Biology 415(4): 716-726. http://dx.doi.org/10.1016/j.jmb.2011.11.037. (Journal Article)
  • Huisman, F.H.A., Koon, N., Bulloch, E.M.M., Baker, H.M., Baker, E.N., Squire, C.J. and Parker, E.J. (2012) Removal of the C-Terminal Regulatory Domain of α-Isopropylmalate Synthase Disrupts Functional Substrate Binding. Biochemistry 51(11): 2289-2297. http://dx.doi.org/10.1021/bi201717j. (Journal Article)
  • Harrison, A.N., Reichau, S. and Parker, E.J. (2012) Synthesis and evaluation of tetrahedral intermediate mimic inhibitors of 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Bioorganic & Medicinal Chemistry Letters 22(2): 907-911. http://dx.doi.org/10.1016/j.bmcl.2011.12.025. (Journal Article)
  • Jiao, W. and Parker, E.J. (2012) Using a Combination of Computational and Experimental Techniques to Understand the Molecular Basis for Protein Allostery. In C. Christov and T. Karabencheva (Ed.), Advances in Protein Chemistry and Structural Biology Vol 87: Structural and Mechanistic Enzymology - Bringing Together Experiments and Computing: 391-413. Oxford: Elsevier. http://dx.doi.org/10.1016/B978-0-12-398312-1.00013-5. (Chapter in Book)
  • Parker, E.J. (2011) Allosteric regulation of the shikimate pathway. Waterville Valley, New Hampshire, USA: Gordon Research Conference: Enzymes, Coenzymes & Metabolic Pathways, 10-15 Jul 2011. (Conference Contribution - Oral presentation)
  • Parker, E.J. and Pratt, A.J. (2011) Amino Acid Biosynthesis. In A.B. Hughes (Ed.), Building Blocks, Catalysis and Coupling Chemistry. Amino Acids, Peptides and Proteins in Organic Chemistry Vol 3: 1-82. Weinheim: Wiley-VCH. http://dx.doi.org/10.1002/9783527631803.ch1. (Chapter in Book)
  • Allison, T.M., Hutton, R.D., Jiao, W., Gloyne, B.J., Nimmo, E.B., Jameson, G.B. and Parker, E.J. (2011) An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Biochemistry 50(43): 9318-9327. http://dx.doi.org/10.1021/bi201231e. (Journal Article)
  • Parker,E.J. (2011) Diverging Mechanisms for the inhibition of a Crucial Biosynthetic Aldolase. Rotorua, New Zealand: Crystal 27 SCANZ Conference, 27-30 Apr 2011. (Conference Contribution - Oral presentation)
  • Parker, E.J. (2011) Enzyme Dynamics and Allosteric Signal Transduction: Examining the Large- and Small-Scale Protein Motions. Hamilton, New Zealand: New Zealand Institute of Chemistry Conference, 27 Nov-1 Dec 2011. (Conference Contribution - Oral presentation)
  • Negron, L., Patchett, M.L. and Parker, E.J. (2011) Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus. Enzyme Research 2011: 134893. http://dx.doi.org/10.4061/2011/134893. (Journal Article)
  • Negron, L. and Parker, E.J. (2011) Fluorinated substrates result in variable leakage of a reaction intermediate during catalysis by dehydroquinate synthase. Organic & Biomolecular Chemistry 9(8): 2861-2867. http://dx.doi.org/10.1039/C0OB01141J. (Journal Article)
  • Tran, D., Pietersma, A.L., Schofield, L.R., Rost, M., Jameson, G.B. and Parker, E.J. (2011) Investigating the role of the hydroxyl groups of substrate erythrose 4-phosphate in the reaction catalysed by the first enzyme of the shikimate pathway. Bioorganic & Medicinal Chemistry Letters 21(22): 6838-6841. http://dx.doi.org/10.1016/j.bmcl.2011.09.017. (Journal Article)
  • Reichau, S., Jiao, W-T., Walker, S.R., Hutton, R.D., Baker, E.N. and Parker, E.J. (2011) Potent inhibitors of a shikimate pathway enzyme from Mycobacterium tuberculosis: Combining mechanism- and modeling-based design. Journal of Biological Chemistry 286(18): 16197-16207. http://dx.doi.org/10.1074/jbc.M110.211649. (Journal Article)
  • Walker, S.R., Jiao, W. and Parker, E.J. (2011) Synthesis and evaluation of dual site inhibitors of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase. Bioorganic & Medicinal Chemistry Letters 21(17): 5092-5097. http://dx.doi.org/10.1016/j.bmcl.2011.03.071. (Journal Article)
  • Allison, T.M., Hutton, R.D., Cochrane, F.C., Yeoman, J.A., Jameson, G.B. and Parker, E.J. (2011) Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Biochemistry 50(18): 3686-3695. http://dx.doi.org/10.1021/bi200251f. (Journal Article)
  • Cross P.J., Dobson R.C.J. and Patchett M.L., Parker E.J. (2011) Tyrosine Latching of a Regulatory Gate Affords Allosteric Control of Aromatic Amino Acid Biosynthesis. The Journal of Biological Chemistry 286(12): 10216-10224. http://dx.doi.org/10.1074/jbc.M110.209924. (Journal Article)
  • Parker, E.J. (2010) Essential TB enzymes as drug targets. Auckland, New Zealand: New Zealand 'Superbugs' Symposium, 16 Nov 2010. (Conference Contribution - Oral presentation)
  • Parker, E.J. (2010) Exploring the evolutionary relationships and the reaction chemistry in a family of crucial biosynthetic aldolases. St Andrews, UK: Royal Society of Chemistry Prizes and Awards Symposium, 28 Apr 2010. (Conference Contribution - Oral presentation)
  • Jiao, W.T., McDonald, Q., Coxon, J.M. and Parker, E.J. (2010) Molecular Modeling Studies of Peptide Inhibitors Highlight the Importance of Conformational Prearrangement for Inhibition of Calpain. Biochemistry 49(26): 5533-5539. http://dx.doi.org/10.1021/bi100048y. (Journal Article)
  • Parker, E.J. (2010) Natural and synthetic inhibitors of key biosynthetic aldolases. Queenstown, New Zealand: Queenstown Molecular Biology Drug Discovery and Development Satellite (QMB), 2-3 Sep 2010. (Conference Contribution - Oral presentation)
  • Parker, E.J. (2010) Natural and synthetic inhibitors of key biosynthetic aldolases. Melbourne, Australia: Royal Australian Chemical Institute (RACI) Convention, 4-8 Jul 2010. (Conference Contribution - Oral presentation)
  • Parker, E.J. (2010) Probing the mechanistic subtleties of a family of crucial biosynthetic aldolases. Honolulu, HI, USA: Pacifichem 2010, 15-20 Dec 2010. (Conference Contribution - Oral presentation)
  • Allison, T.M., Yeoman, J.A., Hutton, R.D., Cochrane, F.C., Jameson, G.B. and Parker, E.J. (2010) Specificity and mutational analysis of the metal-dependent 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Acidithiobacillus ferrooxidans. Biochimica Et Biophysica Acta (BBA) - Proteins & Proteomics 1804(7): 1526-1536. http://dx.doi.org/10.1016/j.bbapap.2010.04.005. (Journal Article)
  • Webby, C.J., Jiao, W., Hutton, R.D., Blackmore, N.J., Baker, H.M., Baker, E.N., Jameson, G.B. and Parker, E.J. (2010) Synergistic Allostery, a Sophisticated Regulatory Network for the Control of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis. Journal of Biological Chemistry 285(40): 30567-30576. http://dx.doi.org/10.1074/jbc.M110.111856. (Journal Article)
  • Huisman, F.H.A., Hunter, M.F.C., Devenish, S.R.A., Gerrard, J.A. and Parker, E.J. (2010) The C-terminal regulatory domain is required for catalysis by Neisseria meningitidis α-isopropylmalate synthase. Biochemical and Biophysical Research Communications 393(1): 168-173. http://dx.doi.org/10.1016/j.bbrc.2010.01.114. (Journal Article)
  • Devenish, S.R.A., Huisman, F.H.A., Parker, E.J., Hadfield, A.T. and Gerrard, J.A. (2009) Cloning and characterisation of dihydrodipicolinate synthase from the pathogen Neisseria meningitidis. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 1794: 1168-1174. http://dx.doi.org/10.1016/j.bbapap.2009.02.003. (Journal Article)
  • Cochrane, F.C., Cookson, T.V.M., Jameson, G.B. and Parker, E.J. (2009) Reversing Evolution: Re-establishing Obligate Metal Ion Dependence in a Metal-independent KDO8P Synthase. Journal of Molecular Biology 390(4): 646-661. http://dx.doi.org/10.1016/j.jmb.2009.05.014. (Journal Article)
  • Walker, S.R., Cumming, H. and Parker, E.J. (2009) Substrate and reaction intermediate mimics as inhibitors of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Organic & Biomolecular Chemistry 7: 3031-3035. http://dx.doi.org/10.1039/b909241b. (Journal Article)
  • Ahn, M., Cochrane, F.C., Patchett, M.L. and Parker, E.J. (2008) Arabinose 5-phosphate analogues as mechanistic probes for Neisseria meningitidis 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Bioorganic & Medicinal Chemistry 16(22): 9830-9836. http://dx.doi.org/10.1016/j.bmc.2008.09.056. (Journal Article)
  • Parker, E.J. (2008) Design, synthesis and evaluation of inhibitors of two key biosynthetic enzymes. Dunedin, New Zealand: Chemistry and the Biosphere Conference, 30 Nov-4 Dec 2008. (Conference Contribution - Oral presentation)
  • Parker, E.J. (2008) Evolving enzymes: Deciphering the evolutionary relationships in a family of crucial biosynthetic aldolases. Dunedin, New Zealand: Chemistry and the Biosphere Conference, 30 Nov-4 Dec 2008. (Conference Contribution - Oral presentation)
  • Parker, E.J. (2008) Evolving enzymes: Deciphering the evolutionary relationships in a family of crucial biosynthetic aldolases. Australian National University, Australia: Invited seminar, 13 Nov 2008. (Oral Presentation)
  • Parker, E.J. (2008) Re-establishing a metal-binding scaffold in a metal-independent KDO8P synthase. Lorne, Australia: 33rd Lorne Conference on Protein Structure and Function, 10-14 Feb 2008. (Conference Contribution - Poster presentation)
  • Parker, E. (2008) Reversing evolution: re-establishing obligate metal-ion dependency in a metal-independent KDO8P synthase. Queenstown, New Zealand: The 18th Queenstown Molecular Biology Meetings, 31 Aug-6 Sep 2008. (Conference Contribution - Oral presentation)
  • Saikia, S., Nicholson, M.J., Young, C., Parker, E.J. and Scott, B. (2008) The genetic basis for indole-diterpene chemical diversity in filamentous fungi. Mycological Research 112(2): 184-199. http://dx.doi.org/10.1016/j.mycres.2007.06.015. (Journal Article)
  • Saikia, S., Nicholson, M. J., Young, C., Parker, E. J. and Scott, B. (2008) The genetic basis for indole-diterpene chemical diversity in filamentous fungi. Mycology research 112: 184-199. (Journal Article)
  • Walter, M., Ford, C.I., Guthrie, J.M., Boyd-Wilson, K.S.H., Di, H.J., Cameron, K.C. and Parker, E.J. (2007) Bioremediation of Aged Pentachlorophenol Residues by Trametes spp.. In M. Rai (Ed.), Mycotechnology: Present Status and Future Prospects: 18-54. New Delhi: I.K. International. (Chapter in Book)
  • Saikia, S., Parker, E.J., Koulman, A. and Scott, B. (2007) Defining Paxilline Biosynthesis in Penicillium paxilli - Functional characterization of two cytochrome p450 monooxygenases. Journal of Biological Chemistry 282(23): 16829-16837. http://dx.doi.org/10.1074/jbc.M701626200. (Journal Article)
  • Parker, E.J., Webby, C.J., Pietersma, A.L., Ahn, M., Cochrane, F.C., Jameson, G.B. and Schofield, R. (2007) Evolving enzymes: Exploring the interplay of sequence structure and reaction chemistry. Lorne, Australia: 32nd Lorne Conference on Protein Structure and Function, 4-8 Feb 2007. (Conference Contribution - Poster presentation)
  • Parker, E.J. (2007) Mechanistic divergence and inhibition of a family of crucial biosynthetic enzymes (Invited). Adelaide, Australia: 22nd Royal Australian Chemical Institute Organic Conference (22RACIOC), 28 Jan-2 Feb 2007. (Conference Contribution - Oral presentation)
  • Scott, B., Young, C.A., Tanaka, A. and Parker, E.J. (2007) Molecular and genetic analysis of symbiosis expressed secondary metabolite genes from the mutualistic fungal endophytes Neotyphodium lolii and Epichloë festucae. In G.D. Robson, P. Van West and G.M. Gadd (Ed.), British Mycological Society Symposia Vol 26: Exploitation of Fungi: 59-77. Cambridge: Cambridge University Press. (Chapter in Book)
  • Walker, S.R. and Parker, E.J. (2006) Design and Synthesis of inhibitors of the DAH7P synthase reaction. Rotorua, New Zealand: NZIC Conference 2006 "Back to the Basics: From Small Molecules to Materials and Surfaces", 2-6 Dec 2006. In Proceedings of the NZIC 2006 II - Posters 109. (Conference Contribution - Poster presentation)
  • Parker, E.J. (2006) Evolving Chemistry: Mechanistic and regulatory divergence in a family of crucial biosynthetic enzymes. Rotorua, New Zealand: NZIC Conference 2006 "Back to the Basics: From Small Molecules to Materials and Surfaces", 2-6 Dec 2006. In Proceedings of the NZIC 2006 I - Talks 31. (Conference Contribution - Oral presentation)
  • Saikia, S., Parker, E.J., Koulman, A. and Scott, B. (2006) Four gene products are required for the fungal synthesis of the indole-diterpene, paspaline. FEBS Letters 580(6): 1625-1630. http://dx.doi.org/10.1016/j.febslet.2006.02.008. (Journal Article)
  • Harrison, A.N. and Parker, E.J. (2006) Inhibitor Analogue Studies on KDO8P Synthase. Rotorua, New Zealand: NZIC Conference 2006 "Back to the Basics: From Small Molecules to Materials and Surfaces", 2-6 Dec 2006. In Proceedings of the NZIC 2006 II - Posters 35. (Conference Contribution - Poster presentation)
  • Bryan, G.T., Johnson, R.D., Parker, E.J., Scott, D.B. and Tanaka, A. (2006) Peramine Biosynthesis. Patent No. 540677, New Zealand. (Intellectual Property)
  • Cochrane, F.C., Patchett, M.L., Jameson, G.B. and Parker, E.J. (2006) Re-establishing a metal-binding scaffold in a metal-independent KDO8P synthase. Rotorua, New Zealand: NZIC Conference 2006 "Back to the Basics: From Small Molecules to Materials and Surfaces", 2-6 Dec 2006. In Proceedings of the NZIC 2006 II - Posters 25. (Conference Contribution - Poster presentation)
  • Cumming, H. and Parker, E.J. (2006) Substrate Analogues to Probe the Mechanisms of two Bacterial Enzymes. Rotorua, New Zealand: NZIC Conference 2006 "Back to the Basics: From Small Molecules to Materials and Surfaces", 2-6 Dec 2006. In Proceedings of the NZIC 2006 II - Posters 26. (Conference Contribution - Poster presentation)
  • Walker, S.R. and Parker, E.J. (2006) Synthesis and evaluation of a mechanism-based inhibitor of a 3-deoxy-D-arabino heptulosonate 7-phosphate synthase. Bioorganic & Medicinal Chemistry Letters 16(11): 2951-2954. http://dx.doi.org/10.1016/j.bmcl.2006.02.073. (Journal Article)
  • Tanaka, A., Tapper, B., Popay, A., Parker, E.J. and Scott, B. (2005) A symbiosis expressed non-ribosomal peptide synthetase from a mutualistic fungal endophyte of perennial ryegrass confers protection to the symbiotum from insect herbivory. Molecular Microbiology 57: 1036-1050. http://tinyurl.com/jv8cb. (Journal Article)
  • Ahn, M., Pietersma, A.L., Schofield, L.R. and Parker, E.J. (2005) Mechanistic divergence of two closely related aldol-like enzyme-catalysed reactions. Organic and Biomolecular Chemistry 3: 4046-4049. http://www.rsc.org/ej/OB/2005/b511661a.pdf. (Journal Article)
  • Williamson, R.M., Pietersma, A.L., Jameson, G.B. and Parker, E.J. (2005) Stereospecific deuteration of 2-deoxyerythrose 4-phosphate using 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Bioorganic and Medicinal Chemistry Letters 15: 2339-2342. http://tinyurl.com/etctt. (Journal Article)
  • Schofield, L.R., Anderson, B.F., Patchett, M.L., Norris, G.E., Jameson, G.B. and Parker, E.J. (2005) Substrate ambiguity and crystal structure of Pyrococcus furiosus 3-deoxy-D arabino-heptulosonate -7- phosphate synthase: An ancestral 3-deoxyald-2-ulosonate-phosphate synthase? Biochemistry 44: 11950-11962. (Journal Article)
  • Webby, C.J., Baker, H.M., Lott, J.S., Baker, E.N. and Parker, E.J. (2005) The structure of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from mycobacterium tuberculosis reveals a common catalytic scaffold and ancestry for type I and type II enzymes. Journal of Molecular Biology 354: 927-939. (Journal Article)
  • Schofield, L.R., Patchett, M.L. and Parker, E.J. (2004) Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus. Protein Expression & Purification 34(1): 17-27. http://tinyurl.com/p7j6o. (Journal Article)
  • Bulloch, E.M.M., Jones, M.A., Parker, E.J., Osborne, A.P., Stephens, E., Davies, G.M., Coggins, J.R. and Abell, C. (2004) Identification of 4-amino-4-deoxychorismate synthase as the molecular target for the antimicrobial action of (6S)-6-fluoroshikimate. Journal of the American Chemical Society 126(32): 9912-9913. http://pubs.acs.org/cgi-bin/article.cgi/jacsat/2004/126/i32/pdf/ja048312f.pdf. (Journal Article)
  • McMillan, L.K., Carr, R.L., Young, C.A., Astin, J.W., Lowe, R.G.T., Parker, E.J., Jameson, G.B., Finch, S.C., Miles, C.O., McManus, O.B., Schmalhofer, W.A., Garcia, M.L., Kacrozowski, G.J., Goetz, M., Tkacz, J.S. and Scott, B. (2003) Molecular analysis of two cytochrome P450 monooxygenase genes required for paxilline biosynthesis in Penicillium paxilli, and effects of paxilline intermediates on mammalian maxi-K ion channels. Molecular Genetics and Genomics 270(1): 9-23. http://www.springerlink.com/media/5n5d6glwth0qw4kmexej/contributions/h/d/b/u/hdbuawleud12aqhg.pdf. (Journal Article)
  • Walter, M., Guthrie, J.M., Sivakumaran, S., Parker, E.J., Slade, A., McNaughton, D. and Boyd-Wilson, K.S.H. (2003) Screening of New Zealand native white-rot isolates for PCP degradation. Bioremediation Journal 7(2): 119-128. (Journal Article)
  • Parker, E.J., Bello, C.G., Coggins, J.R., Hawkins, A.R. and Abell, C. (2000) Mechanistic studies on type I and type II dehydroquinase with (6R)- and (6S)-6-fluoro-3-dehydroquinic acids. Bioorganic & Medicinal Chemistry Letters 10(3): 231-234. http://tinyurl.com/zjwrh. (Journal Article)
  • Frederickson M, Parker EJ., Hawkins AR, Coggins JR. and Abell C. (2000) Selective Inhibition of Type II Dehydroquinases. Journal of Organic Chemistry 64(8): 2612-2613. (Journal Article)
  • Osborne A.P., Parker E., J.. and Abell C. (1999) Biotransformations to generate fluorinated analogues of shikimate pathway intermediates. Fluorinated Bioact Compd Agric Med Fields : 13/1-13/10. (Journal Article)
  • Parker E.J., Coggins J.R. and Abell C. (1997) Derailing dehydroquinate synthase by introducing a stabilising stereoelectronic effect in a reaction intermediate. Journal of Organic Chemistry 62: 8582-8585. (Journal Article)
  • Peter J. Duggan, Emily Parker., John Coggins. and Chris Abell. (1995) ENZYMATIC SYNTHESIS OF (6R) AND (6S)-FLUOROSHIKIMIC ACIDS. Bioorganic and medicinal chemistry letters 5(20): 2347-2352. (Journal Article)
  • Abell AD, Hoult DA. and Jamieson EJ. (1992) Synthesis of 1,2-disubstituted pyrroles: a cis peptide bond surrogate. Tetrahedron Letters 33: 5831-5832. (Journal Article)